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PNGase F from Elizabethkingia miricola

Enzyme Commission (EC) Number  3.2.1.52  ( BRENDA | IUBMB )
Properties
Price and Availability
formliquid
specific activity
activity
25 units/100 μL protein
ml wtMᵣ = 62 kDa
Manufacturerqlyco Ltd.
packaging300 μL of buffered aqueous solution
optimum pH5.0 - 5.5
shipped insent at 4°C
Pack Size
Availability
Price
(RMB)
1 vial
>10 left in stock
(Nanjing)
995.0
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Description

Bulk Ordering and Pricing:
Application
PNGase F (Peptide-N-glycosidase F is one of the most widely used enzymes for the deglycosylation of glycoproteins. It occurs in the culture fluid of the pathogenic microorganism Elizabethkingia meningosepticum. The enzyme releases asparagine-linked oligosaccharides from glycoproteins and glycopeptides by hydrolyzing the amide of the asparagine (Asn) side chain. A tripeptide with the oligosaccharide-linked asparagine as the central residue is the minimal substrate for PNGase F. The oligosaccharides can be high mannose, hybrid, or complex type. However, N-glycans with fucose linked alpha(1-3) to the Asn-bound N-acetylglucosamine are resistant to the action of PNGase F.
General description
N-Glycosidase F, Peptide N-glycosidase
Other Notes
For life science research only. Not for use in diagnostic procedures.
Preparation Note
Working concentration:
Enzyme/substrate ratio should be in the range of 0.04 U/25-80 μg.
Specificity
Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.
Physical form
300 μL enzyme solution.

Larger quantities for your development, manufacturing or research applications needed?

Please enquire at bulk@qlyco.com

Safety Information

Documents
Safety Information for this product is unavailable at this time.
Datasheet Pngase F

Protocols

Technical Service
No protocol yet available
Our team has experience in all areas of research including Life Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact us at techservices@qlyco.com
 
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